Anti-Prostate Specific Antigen antibody [8301] (ab403)
![Anti-Prostate Specific Antigen antibody [8301] (ab403)](https://www.abcam.com/ps/products/0/ab403/Images/ab403-3501-anti-prostate-specific-antigen-antibody-8301-immunohistochemistry.jpg "Anti-Prostate Specific Antigen antibody [8301] (ab403)")
Key features and details
- Mouse monoclonal [8301] to Prostate Specific Antigen
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Prostate Specific Antigen antibody [8301]
See all Prostate Specific Antigen primary antibodies -
Description
Mouse monoclonal [8301] to Prostate Specific Antigen -
Host species
Mouse -
Specificity
Human prostate-specific antigen - 100% PSA/alpha-1-ACT-complex - 59% Kallikrein - 11 l/mol human prostate-specific antigen. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
native protein (purified), corresponding to amino acids 158-163 of Human Prostate Specific Antigen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.00
Preservative: 0.095% Sodium azide
Constituents: 0.335% PBS, 1.0878% Sodium citrate, 0.9% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
8301 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [8301] (ab403)Human normal prostate. Staining is localised as cytoplasmic and secreted. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system, at room temperature: sections were rehydrated and antigen retrieved with buffers (citrate pH 6.1) in a PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
