Human U-Plasminogen Activator ELISA Kit (ab226904)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human U-Plasminogen Activator ELISA Kit
See all Urokinase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Urine 5 1.7% Inter-assay Sample n Mean SD CV% Urine 3 2.6% -
Sample type
Cell culture supernatant, Urine, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 108 96% - 113% Urine 106 97% - 115% Serum 87 85% - 90% Cell culture extracts 84 83% - 86% Hep Plasma 87 86% - 88% EDTA Plasma 91 88% - 93% Cit plasma 89 89% - 90% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human U-Plasminogen Activator ELISA Kit (ab226904) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of U-Plasminogen Activator protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human U-Plasminogen Activator with 4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml 10X Human U-Plasminogen Activator Capture Antibody 1 x 600µl 10X Human U-Plasminogen Activator Detector Antibody 1 x 600µl Human U-Plasminogen Activator Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
Tissue specificity
Expressed in the prostate gland and prostate cancers. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsPhosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ATF
- ATF uPA
- BDPLT5
see all -
Database links
- Entrez Gene: 5328 Human
- Omim: 191840 Human
- SwissProt: P00749 Human
- Unigene: 77274 Human
Images
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Other - Human U-Plasminogen Activator ELISA Kit (ab226904)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Human U-Plasminogen Activator standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of Human U-Plasminogen Activator standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native U-Plasminogen Activator in Human serum and plasma samplesThe concentrations of U-Plasminogen Activator were measured in duplicates, interpolated from the U-Plasminogen Activator standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (heparin) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean U-Plasminogen Activator concentration was determined to be 439 pg/mL in serum, 732 pg/mL in plasma (citrate), 678 pg/mL in plasma (heparin), and 871 pg/ml in plasma (EDTA).
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Interpolated concentrations of spiked U-Plasminogen Activator in Human serum and plasma samplesThe concentrations of U-Plasminogen Activator were measured in duplicates, interpolated from the U-Plasminogen Activator standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Serum from ten individual healthy Human male donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean U-Plasminogen Activator concentration was determined to be 500 pg/mL with a range of 265 – 745 pg/mL.
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Interpolated concentrations of native U-Plasminogen Activator in Human HEPG2 and PC-3 cell culture supernatant and urine samplesThe concentrations of U-Plasminogen Activator were measured in duplicates, interpolated from the U-Plasminogen Activator standard curves and corrected for sample dilution. Undiluted samples are as follows: HepG2 supernatant 25%, PC-3 supernatant 1:250, and Urine 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean U-Plasminogen Activator concentration was determined to be 10 ng/mL in HepG2 supernatant, 865 ng/mL in PC-3 supernatant, and 73 ng/mL in Urine.
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Interpolated concentrations of native U-Plasminogen Activator in Human PC-3 cell extract based on a 31.3 µg/mL extract loadThe concentrations of U-Plasminogen Activator were measured in duplicate and interpolated from the U-Plasminogen Activator standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean U-Plasminogen Activator concentration was determined to be 4689 pg/mL in PC-3 Cell Extract.
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Sandwich ELISA - Human U-Plasminogen Activator ELISA Kit (ab226904)To learn more about the advantages of recombinant antibodies see here.
