Anti-Nicastrin antibody (ab45425)
Key features and details
- Rabbit polyclonal to Nicastrin
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Nicastrin antibody
See all Nicastrin primary antibodies -
Description
Rabbit polyclonal to Nicastrin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Nicastrin.
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Positive control
- Mouse brain tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nicastrin antibody (ab45425)Lane 1 : Anti-Nicastrin antibody (ab45425) at 0.5 µg/ml
Lane 2 : Anti-Nicastrin antibody (ab45425) at 1 µg/ml
All lanes : Mouse brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti-rabbit HRP at 1/10000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDa
Additional bands at: 54 kDa, 58 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicastrin antibody (ab45425)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling Nicastrin with Anti-Nicastrin antibody (ab45425) at 20μg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicastrin antibody (ab45425)ab45425 at 2.5 µg/ml staining Nicastrin in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
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Immunocytochemistry/ Immunofluorescence - Anti-Nicastrin antibody (ab45425)Immunofluorescence of Nicastrin in Human Brain cells using ab45425 at 20 ug/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicastrin antibody (ab45425) This image is courtesy of an Abreview submitted by Carl Hobbsab45425 staining Nicastrin in Human breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2500 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
