Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Nicastrin antibody [EPR2575Y] (ab68145)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2575Y] to Nicastrin
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Nicastrin antibody [EPR2575Y]
    See all Nicastrin primary antibodies

  • Description

    Rabbit monoclonal [EPR2575Y] to Nicastrin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Nicastrin aa 650-750 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HEK293T whole cell lysate, HeLa whole cell lysate (ab150035), HL-60 cell lysate, Jurkat whole cell lysate (ab7899)

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Nicastrin antibody [EPR2575Y] (ab68145)
    Western blot - Anti-Nicastrin antibody [EPR2575Y] (ab68145)
    All lanes : Anti-Nicastrin antibody [EPR2575Y] (ab68145) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : NCSTN knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HL-60 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 78 kDa
    Observed band size: 100-125 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab68145 observed at 100-125 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab68145 Anti-Nicastrin antibody [EPR2575Y] was shown to specifically react with Nicastrin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266602 (knockout cell lysate ab258061) was used. Wild-type and Nicastrin knockout samples were subjected to SDS-PAGE. ab68145 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Nicastrin antibody [EPR2575Y] (ab68145)
    Western blot - Anti-Nicastrin antibody [EPR2575Y] (ab68145)
    All lanes : Anti-Nicastrin antibody [EPR2575Y] (ab68145) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HL-60 cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 78 kDa
    Additional bands at: 100-120 kDa (possible isoform), 130-160 kDa (possible isoform)

  • Anti-Nicastrin antibody [EPR2575Y] (ab68145)
    Anti-Nicastrin antibody [EPR2575Y] (ab68145)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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