Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Nicastrin antibody [EPR2575Y] (ab68145)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2575Y] to Nicastrin
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-Nicastrin antibody [EPR2575Y]
See all Nicastrin primary antibodies
Description

Rabbit monoclonal [EPR2575Y] to Nicastrin
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Nicastrin aa 650-750 (C terminal). The exact sequence is proprietary.
Positive control
- WB: HEK293T whole cell lysate, HeLa whole cell lysate (ab150035), HL-60 cell lysate, Jurkat whole cell lysate (ab7899)
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2575Y
Isotype

IgG
Research areas

Western blot - Anti-Nicastrin antibody [EPR2575Y] (ab68145)

All lanes : Anti-Nicastrin antibody [EPR2575Y] (ab68145) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : NCSTN knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HL-60 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 78 kDa
Observed band size: 100-125 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab68145 observed at 100-125 kDa. Red - loading control ab8245 observed at 36 kDa.

ab68145 Anti-Nicastrin antibody [EPR2575Y] was shown to specifically react with Nicastrin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266602 (knockout cell lysate ab258061) was used. Wild-type and Nicastrin knockout samples were subjected to SDS-PAGE. ab68145 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Nicastrin antibody [EPR2575Y] (ab68145)

All lanes : Anti-Nicastrin antibody [EPR2575Y] (ab68145) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HL-60 cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP conjugated Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 78 kDa
Additional bands at: 100-120 kDa (possible isoform), 130-160 kDa (possible isoform)
Anti-Nicastrin antibody [EPR2575Y] (ab68145)

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