Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CLN5 antibody [EPR12197(B)] (ab170899)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12197(B)] to CLN5
Suitable for: ICC, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-CLN5 antibody [EPR12197(B)]
See all CLN5 primary antibodies
Description

Rabbit monoclonal [EPR12197(B)] to CLN5
Host species

Rabbit
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human CLN5 aa 150-250 (Cysteine residue). The exact sequence is proprietary.
Database link: O75503
(Peptide available as ab204069)
Positive control
- WB: Fetal kidney tissue lysate. T47-D, A431, NIH/3T3, A549, and HepG2 whole cell lysates; IHC: Human kidney and liver tissue; ICC: A431 and T-47D cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12197(B)
Isotype

IgG
Research areas

Western blot - Anti-CLN5 antibody [EPR12197(B)] (ab170899)

All lanes : Anti-CLN5 antibody [EPR12197(B)] (ab170899) at 1/1000 dilution (purified)

Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 41 kDa
Observed band size: 48-52 kDa
why is the actual band size different from the predicted?

The molecular weights observed are consistent with what have been described in the literature (PMID: 26342652).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLN5 antibody [EPR12197(B)] (ab170899)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling CLN5 with purified ab170899 at 1/2000 dilution (0.22 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry - Anti-CLN5 antibody [EPR12197(B)] (ab170899)

Immunocytochemistry/ Immunofluorescence analysis of T-47D (Human ductal breast epithelial tumor epithelial cell) cells labeling CLN5 with purified ab170899 at 1/50 dilution (8.7 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-CLN5 antibody [EPR12197(B)] (ab170899)

Anti-CLN5 antibody [EPR12197(B)] (ab170899) at 1/5000 dilution (purified) + C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 41 kDa
Observed band size: 48-52 kDa why is the actual band size different from the predicted?

The molecular weights observed are consistent with what have been described in the literature (PMID: 26342652).
Western blot - Anti-CLN5 antibody [EPR12197(B)] (ab170899)

All lanes : Anti-CLN5 antibody [EPR12197(B)] (ab170899) at 1/1000 dilution (unpurified)

Lane 1 : Fetal kidney lysate
Lane 2 : T47-D cell lysate
Lane 3 : A431 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 41 kDa
Immunocytochemistry - Anti-CLN5 antibody [EPR12197(B)] (ab170899)

Immunofluorescent analysis of A431 cells labeling CLN5 with unpurified ab170899 at 1/100 dilution (green). DAPI nuclear staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLN5 antibody [EPR12197(B)] (ab170899)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CLN5 with unpurified ab170899 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-CLN5 antibody [EPR12197(B)] (ab170899)

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