Anti-Nicastrin antibody - C-terminal (ab226299)
Key features and details
- Rabbit polyclonal to Nicastrin - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nicastrin antibody - C-terminal
See all Nicastrin primary antibodies -
Description
Rabbit polyclonal to Nicastrin - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human Nicastrin aa 659-709 (C terminal). The exact sequence is proprietary. (NP_056146.1).
Database link: Q92542 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226299 was affinity purified using an epitope specific to Nicastrin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nicastrin antibody - C-terminal (ab226299)Anti-Nicastrin antibody - C-terminal (ab226299) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate prepared using RIPA buffer at 50 µg
Developed using the ECL technique.
Predicted band size: 78 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-Nicastrin antibody - C-terminal (ab226299)Nicastrin was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using RIPA lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226299 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226299 at 1 µg/ml.
Lane 1: ab226299 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
