Anti-BACE1 antibody (ab63954)
Key features and details
- Rabbit polyclonal to BACE1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-BACE1 antibody
See all BACE1 primary antibodies -
Description
Rabbit polyclonal to BACE1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
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Positive control
- This antibody gave a positive signal in the following lysates: Rat Lung Tissue HepG2 Whole Cell Mouse Kidney Tissue Human Liver Tissue (Data not shown)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatHumanAll applications Macaque monkeyApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 56, 70 kDa (predicted molecular weight: 56 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 56, 70 kDa (predicted molecular weight: 56 kDa).Target
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Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. -
Tissue specificity
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. -
Sequence similarities
Belongs to the peptidase A1 family. -
Domain
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 23621 Human
- Entrez Gene: 23821 Mouse
- Entrez Gene: 29392 Rat
- Omim: 604252 Human
- SwissProt: P56817 Human
- SwissProt: P56818 Mouse
- SwissProt: P56819 Rat
- Unigene: 504003 Human
see all -
Alternative names
- APP beta secretase antibody
- Asp 2 antibody
- ASP2 antibody
see all
Images
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Western blot - Anti-BACE1 antibody (ab63954)All lanes : Anti-BACE1 antibody (ab63954) at 1 µg/ml
Lane 1 : Lung (Rat) Tissue Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56,70 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
BACE1 is known to run at 70 kDa due to potential glycosolation sites. It is beleived the 56 kDa band may correspond to the unglycosylated form of the protein.
Datasheets and documents
References (0)
ab63954 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-BACE1 antibody (ab63954)All lanes : Anti-BACE1 antibody (ab63954) at 1 µg/ml
Lane 1 : Lung (Rat) Tissue Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56,70 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
BACE1 is known to run at 70 kDa due to potential glycosolation sites. It is beleived the 56 kDa band may correspond to the unglycosylated form of the protein.
