Anti-Caspase-12 antibody (ab62484)
Key features and details
- Rabbit polyclonal to Caspase-12
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Caspase-12 antibody
See all Caspase-12 primary antibodies -
Description
Rabbit polyclonal to Caspase-12 -
Host species
Rabbit -
Specificity
This antibody reacts with both the propeptide and cleaved forms of Caspase-12.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse Caspase-12. immunogen sequence from within aa 181 to 240
Database link: O08736 -
Positive control
- WB: Human heart lysate. Rat dorsal root ganglion. IHC-P: Human and rat heart tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Caspase-12 Antibody is (Large)affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-12 antibody (ab62484)
Paraffin-embedded rat heart tissue stained for Caspase-12 using ab62484 at 2.5 µg/ml dilution in immunohistochemical analysis.
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Western blot - Anti-Caspase-12 antibody (ab62484)Lane 1 : Anti-Caspase-12 antibody (ab62484) at 0.5 µg/ml
Lane 2 : Anti-Caspase-12 antibody (ab62484) at 1 µg/ml
Lane 3 : Anti-Caspase-12 antibody (ab62484) at 2 µg/ml
All lanes : Human heart lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti rabbit IgG secondary antibody
Predicted band size: 38-45 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
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Western blot - Anti-Caspase-12 antibody (ab62484) This image is courtesy of an abreview submitted by Karine Thibault, CNRS, Paris, FranceAll lanes : Anti-Caspase-12 antibody (ab62484) at 1/2000 dilution
Lane 1 : rat dorsal root ganglion
Lane 2 : treated rat dorsal root ganglion
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti-rabbit HRP-linked secondary antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38-45 kDa
Exposure time: 2 minutes
The full length Casp12 (42 kDa) observed in control rat was cleaved into an active form with a size of 38 kDa in treated rat -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-12 antibody (ab62484)Paraffin-embedded human heart tissue stained for Caspase-12 using ab62484 at 2 µg/ml dilution in immunohistochemical analysis.
