Anti-Caspase-12 antibody (ab235180)
Key features and details
- Rabbit polyclonal to Caspase-12
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Caspase-12 antibody
See all Caspase-12 primary antibodies -
Description
Rabbit polyclonal to Caspase-12 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseWB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: NIH/3T3 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Caspase-12 antibody (ab235180)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling Caspase-12 with ab235180 at 15 µg/ml , followed by Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 15 µg/ml for 1 hour at RT (green).
Expected localization: Endoplasmic reticulum. The nuclear counter stain is DAPI (blue).
Panel A: DAPI.
Panel B: ab235180 + secondary antibody.
Panel C: Merge of panel A and B.
Panel D: Secondary only.
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Western blot - Anti-Caspase-12 antibody (ab235180)Anti-Caspase-12 antibody (ab235180) at 0.5 µg/ml + NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 35 µg
Secondary
Goat anti-rabbit IgG [H&L] HRP at 1/40000 dilution
Predicted band size: 38 kDa
Exposure time: 30 seconds
