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Anti-AP-2 complex subunit alpha-1 antibody (ab205222)

Key features and details

  • Rabbit polyclonal to AP-2 complex subunit alpha-1
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-AP-2 complex subunit alpha-1 antibody
    See all AP-2 complex subunit alpha-1 primary antibodies

  • Description

    Rabbit polyclonal to AP-2 complex subunit alpha-1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human AP-2 complex subunit alpha-1 aa 600-700. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_055018.2).
    Database link: O95782

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysate (ab7899).

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-AP-2 complex subunit alpha-1 antibody (ab205222)
    Western blot - Anti-AP-2 complex subunit alpha-1 antibody (ab205222)
    All lanes : Anti-AP-2 complex subunit alpha-1 antibody (ab205222) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 108 kDa


    Exposure time: 10 seconds


    Lysates prepared using NETN lysis buffer.

  • Immunoprecipitation - Anti-AP-2 complex subunit alpha-1 antibody (ab205222)
    Immunoprecipitation - Anti-AP-2 complex subunit alpha-1 antibody (ab205222)

    Detection of AP-2 complex subunit alpha-1 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab205222 at 6 µg/mg lysate for IP and at 0.1 µg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

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