Anti-VPS18 antibody - C-terminal (ab229335)
Key features and details
- Rabbit polyclonal to VPS18 - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VPS18 antibody - C-terminal
See all VPS18 primary antibodies -
Description
Rabbit polyclonal to VPS18 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide within Human VPS18 (C terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: Q9P253 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. ICC/IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-VPS18 antibody - C-terminal (ab229335)All lanes : Anti-VPS18 antibody - C-terminal (ab229335) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 110 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody - C-terminal (ab229335)A431 (human epidermoid carcinoma cell line) cells stained for VPS18 (green) using ab229335 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
