Anti-PSD95 antibody (ab12093)
Key features and details
- Goat polyclonal to PSD95
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PSD95 antibody
See all PSD95 primary antibodies -
Description
Goat polyclonal to PSD95 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.025% Sodium azide
Constituents: PBS, 1% BSA
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PSD95 antibody (ab12093)Lanes 1-4 : Anti-PSD95 antibody (ab12093) at 2 µg/ml (Blocked with 2% BSA)
Lanes 5-8 : Anti-PSD95 antibody (ab12093) at 2 µg/ml (Blocked with 3% Milk)
Lanes 1 & 5 : Human brain cerebral cortex tissue lysate - total protein (ab30061)
Lanes 2 & 6 : Human brain tissue lysate - total protein
Lanes 3 & 7 : Brain (Mouse) Tissue Lysate
Lanes 4 & 8 : Brain (Rat) Tissue Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 38 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin or 3% Milk before being incubated with ab12093 overnight at 4°C. Antibody binding was detected using an anti-goat antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody (ab12093) This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdomab12093 PSD95 antibody immunostaining brain tissue (4% PFA perfused paraffin embedded 5 micron saggital section). ab12093 was used at 10 µg/ml for a 20 minute incubation at 37 degrees C (on a Ventana Discovery slide processor). Light antigen retrieval and then light protease treatment is recommended before addition of primary antibody ab12093 in this paradigm. -
Western blot - Anti-PSD95 antibody (ab12093) This image is courtesy of an Abreview submitted by Dr Martin BroadstockAll lanes : Anti-PSD95 antibody (ab12093) at 1/5000 dilution
Lane 1 : rat cortical lysate
Lane 2 : mouse cortical lysate
Lanes 3-8 : BA21 human cortical lane lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexafluor 680
Performed under reducing conditions.
Predicted band size: 80 kDa
See abreview for further details -
Western blot - Anti-PSD95 antibody (ab12093) This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United KingdomLane 1 : Anti-PSD95 antibody (ab12093) at 0.3 µg/ml
Lane 2 : Anti-PSD95 antibody (ab12093) at 1 µg/ml
Lanes 3-4 : Anti-PSD95 antibody (ab12093) at 3 µg/ml
Lanes 1-3 : 10µg mouse brain extract/lane
Lane 4 : 10µg mouse brain extract/lane with Mouse PSD95 peptide (ab12419) at 1 µg/ml
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: ~105 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsPSD95 (ab12093) western blot on mouse brain extract (10
µ g protein/lane), showing use of the immunising peptide (ab12419) as a control. The blot was exposed for 10 seconds to autoradiographic film.Lane 1 = 0.3
µ
Lane 2 = 1µ
Lane 3 = 3µ
Lane 4 = 3µ µ
Lane 5 = Ladder
