Anti-TTC5 antibody (ab36855)
Key features and details
- Rabbit polyclonal to TTC5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TTC5 antibody -
Description
Rabbit polyclonal to TTC5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
A 14 amino acid peptide from near the carboxy terminus of human TTC5.
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Positive control
- RAW264.7 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TTC5 antibody (ab36855)Lane 1 : Anti-TTC5 antibody (ab36855) at 0.5 µg/ml
Lane 2 : Anti-TTC5 antibody (ab36855) at 1 µg/ml
Lane 3 : Anti-TTC5 antibody (ab36855) at 2 µg/ml
All lanes : RAW264.7 cell lysate
Lysates/proteins at 15 µg/ml per lane.
Secondary
All lanes : anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC5 antibody (ab36855)ab36855 at 10µg/ml staining TCC5 in mouse brain tissue by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-TTC5 antibody (ab36855)ICC/IF image of ab36855 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36855, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor®594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Anti-TTC5 antibody (ab36855)Immunofluorescence of TTC5 in Mouse Brain cells using ab36855 at 20 ug/ml.