Anti-VPS18 antibody (ab241131)
Key features and details
- Rabbit polyclonal to VPS18
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-VPS18 antibody
See all VPS18 primary antibodies -
Description
Rabbit polyclonal to VPS18 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide within Human VPS18 aa 900-950. The exact sequence is proprietary.
Database link: Q9P253 -
Positive control
- WB: HeLa, HEK-293T and Jurkat cell lysate. IP: HEK-293T cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-VPS18 antibody (ab241131)All lanes : Anti-VPS18 antibody (ab241131) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutesPrepared using NETN lysis buffer.
-
Immunoprecipitation - Anti-VPS18 antibody (ab241131)VPS18 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241131 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241131 at 1 µg/ml.
Lane 1: ab241131 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 3 minutes.
