Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸ 10 µl 137 366 ₸

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Anti-BACE1 antibody [EPR3956] (ab108394)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3956] to BACE1
Suitable for: WB, IP
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-BACE1 antibody [EPR3956]
See all BACE1 primary antibodies
Description

Rabbit monoclonal [EPR3956] to BACE1
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: SH-SY5Y cell lysate and human fetal, mouse and rat brain tissue lysates. IP: Human fetal brain tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3956
Isotype

IgG
Research areas

Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: BACE1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: SHSY5Y whole cell lysate (20 µg)
Lane 4: Human brain whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab108394 observed at 70 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab108394 was shown to recognize BACE1 in wild type cells as signal was lost at the expected MW in BACE1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BACE1 knockout samples were subjected to SDS-PAGE. Ab108394 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)

All lanes : Anti-BACE1 antibody [EPR3956] (ab108394) at 1/10000 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 70 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)

Anti-BACE1 antibody [EPR3956] (ab108394) at 1/10000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-BACE1 antibody [EPR3956] (ab108394)

ab108394 (purified) at 1/40 immunoprecipitating BACE1 in human fetal brain whole tissue lysate.

Lane 1 (input): human fetal brain whole tissue lysate (10µg)

Lane 2 (+): ab108394 + human fetal brain whole tissue lysate (10µg).

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108394 in human fetal brain whole tissue lysate.

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-BACE1 antibody [EPR3956] (ab108394)

All lanes : Anti-BACE1 antibody [EPR3956] (ab108394) at 1/1000 dilution (unpurified)

Lane 1 : Fetal brain lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 56 kDa
Anti-BACE1 antibody [EPR3956] (ab108394)

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