Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 107 212 ₸

Order now and get it on Tuesday March 09, 2021

Anti-BACE1 antibody [EPR22802-233] (ab263901)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22802-233] to BACE1
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-BACE1 antibody [EPR22802-233]
See all BACE1 primary antibodies
Description

Rabbit monoclonal [EPR22802-233] to BACE1
Host species

Rabbit
Specificity

This antibody is not recommended for human IHC-P
Tested Applications & Species

Application Species
IHC-P
Mouse

Rat

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged human BACE1 recombinant protein; Wild-type HAP1 and SH-SY5Y whole cell lysates; Mouse brain, Human brain and Rat hippocampus lysates. IHC-P: Mouse hippocampus and Rat hippocampus tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Mouse Rat
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22802-233
Isotype

IgG
Research areas

Western blot - Anti-BACE1 antibody [EPR22802-233] (ab263901)

All lanes : Anti-BACE1 antibody [EPR22802-233] (ab263901) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : BACE1 knockout HAP1 whole cell lysate
Lane 3 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 4 : Mouse brain lysate
Lane 5 : Human brain lysate
Lane 6 : Rat hippocampus lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 56 kDa
Observed band size: 70 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lanes 1-2:26 seconds;
Lanes 3-6:10 seconds.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 17425515).

ab263901 was shown to specifically react with BACE1 in wild-type HAP1 cells as signal was lost in BACE1 knockout cells. Wild-type and BACE1 knockout samples were subjected to SDS-PAGE. ab263901 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument using the ECL technique.
Western blot - Anti-BACE1 antibody [EPR22802-233] (ab263901)

Anti-BACE1 antibody [EPR22802-233] (ab263901) at 1/1000 dilution + His-tagged human BACE1 recombinant protein (aa22-460 &aa46-460), 10 ng

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 56 kDa
Observed band size: 65-67,68-70 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] (ab263901)

Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling BACE1 with ab263901 at 1/4000 dilution (0.19 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mossy fibers in the hilar region of the dentate gyrus. The section was incubated with ab263901 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE1 antibody [EPR22802-233] (ab263901)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling BACE1 with ab263901 at 1/4000 dilution (0.19 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mossy fibers in the hilar region of the dentate gyrus. The section was incubated with ab263901 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)..

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Anti-BACE1 antibody [EPR22802-233] (ab263901)

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