Anti-MHC class II I E kappa antibody [14-4-4S] (ab25681)
![Anti-MHC class II I E kappa antibody [14-4-4S] (ab25681)](https://www.abcam.com/ps/products/25/ab25681/Images/ab25681-1-anti-mhc-class-ii-i-e-kappa-antibody-14-4-4s-flow-cytometry-splenocytes-mouse.jpg "Anti-MHC class II I E kappa antibody [14-4-4S] (ab25681)")
Key features and details
- Mouse monoclonal [14-4-4S] to MHC class II I E kappa
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-MHC class II I E kappa antibody [14-4-4S]
See all MHC class II I E kappa primary antibodies -
Description
Mouse monoclonal [14-4-4S] to MHC class II I E kappa -
Host species
Mouse -
Specificity
This antibody is specific to an epitope on mouse I-E kappa MHC class II alloantigen. The antibody reacts with the I-E kappa class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. The antibody has also been reported to cross react with the rat class II alloantigen RT1D.
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Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse MHC class II I E kappa. C3H mouse skin graft and splenocytes
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Positive control
- Flow Cyt: AKR mouse splenocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Constituent: 100% Borate buffered saline
No preservatives or amine-containing buffer salts added. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
14-4-4S -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-MHC class II I E kappa antibody [14-4-4S] (ab25681)
Flow Cytometry analysis of AKR mouse splenocytes stained for MHC class II I E kappa using ab25681 (1 µg/106 cells) and a Rat Anti-Mouse CD3ε-PE followed by a Goat Anti-Mouse IgG2a, Human ads-FITC.
