Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸ 10 µl 127 315 ₸

Order now and get it on Wednesday March 10, 2021

Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22225] to DPEP1/MDP
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-DPEP1/MDP antibody [EPR22225]
See all DPEP1/MDP primary antibodies
Description

Rabbit monoclonal [EPR22225] to DPEP1/MDP
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human kidney and testis lysates. IHC-P: Human kidney, colon cancer and testis tissue.
General notes
This product was previously labelled as DPEP1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22225
Isotype

IgG
Research areas
- Cancer
- Tumor biomarkers
- Enzymes
- Other

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling DPEP1/MDP with ab230978 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in apical surface of human colon cancer (PMID: 28413640; PMID 23839495) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DPEP1/MDP with ab230978 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in basement membrane of human testis is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling DPEP1/MDP with ab230978 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in proximal tubules of human kidney (PMID: 8663190) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

All lanes : Anti-DPEP1/MDP antibody [EPR22225] (ab230978) at 1/1000 dilution

Lane 1 : Human kidney lysate at 20 µg
Lane 2 : Human testis lysate at 10 µg

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 46 kDa
Observed band size: 55 kDa
why is the actual band size different from the predicted?

Exposure time: 50 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 23839495).
Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

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