Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22225] to DPEP1/MDP
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-DPEP1/MDP antibody [EPR22225]
    See all DPEP1/MDP primary antibodies

  • Description

    Rabbit monoclonal [EPR22225] to DPEP1/MDP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human kidney and testis lysates. IHC-P: Human kidney, colon cancer and testis tissue.

  • General notes

     This product was previously labelled as DPEP1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22225

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling DPEP1/MDP with ab230978 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in apical surface of human colon cancer (PMID: 28413640; PMID 23839495) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DPEP1/MDP with ab230978 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in basement membrane of human testis is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling DPEP1/MDP with ab230978 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in proximal tubules of human kidney (PMID: 8663190) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)
    Western blot - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)
    All lanes : Anti-DPEP1/MDP antibody [EPR22225] (ab230978) at 1/1000 dilution

    Lane 1 : Human kidney lysate at 20 µg
    Lane 2 : Human testis lysate at 10 µg

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 50 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 23839495).

  • Anti-DPEP1/MDP antibody [EPR22225] (ab230978)
    Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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