Enterokinase Activity Assay Kit (Fluorometric) (ab170964)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Purified protein
- Sensitivity: 1 µU
Overview
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Product name
Enterokinase Activity Assay Kit (Fluorometric)
See all Enterokinase kits -
Detection method
Fluorescent -
Sample type
Purified protein -
Assay type
Enzyme activity (quantitative) -
Sensitivity
>= 1 µU -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's Enterokinase Activity Assay Kit (Fluorometric) utilizes a peptide substrate containing the Enterokinase recognition sequence along with a fluorescent label ‘AFC’. Enterokinase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. This assay kit is simple and rapid and can detect Enterokinase activity as low as 1 mU.
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Notes
Enterokinase (Enteropeptidase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of Enterokinase has been utilized in cleaving a variety of native or fusion protein tags containing the above recognition motif.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests AFC Standard Yellow 1 x 100µl Enteropeptidase Assay Buffer WM 1 x 20ml Enteropeptidase Substrate Red 1 x 0.2ml Human Enteropeptidase Green 1 x 50µl -
Research areas
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Function
Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. -
Tissue specificity
Intestinal brush border. -
Involvement in disease
Defects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD) [MIM:226200]; a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 2 LDL-receptor class A domains.
Contains 1 MAM domain.
Contains 1 peptidase S1 domain.
Contains 1 SEA domain.
Contains 1 SRCR domain. -
Post-translational
modificationsThe chains are derived from a single precursor that is cleaved by a trypsin-like protease. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- PRSS 7
- PRSS7
- Enterokinase
see all
Images
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AFC Standard Curve
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Measurement of Enterokinase Levels
