Anti-ALS2CR8 antibody [EPR13712] (ab180142)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13712] to ALS2CR8
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-ALS2CR8 antibody [EPR13712]
See all ALS2CR8 primary antibodies -
Description
Rabbit monoclonal [EPR13712] to ALS2CR8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human -
Immunogen
Recombinant fragment within Human ALS2CR8 aa 100-300. The exact sequence is proprietary.
Database link: Q8N187 -
Positive control
- SH-SY5Y and 293T cell lysate, SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13712 -
Isotype
IgG -
Research areas
Images
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Flow cytometric analysis of 2% paraformaldehyde fixed SH-SY5Y cells labeling ALS2CR8 with ab180142 at 1/90. Secondary antibody Goat anti rabbit IgG (FITC) at 1/75. Isotype control Rabbit IgG
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All lanes : Anti-ALS2CR8 antibody [EPR13712] (ab180142) at 1/10000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 81 kDa
Observed band size: 81 kDaBlocking buffer and concentration: 5% NFDM/TBST
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