Antibodies, Proteins, Kits and Reagents for Life Science

341 070 ₸ Product size

100 µl 341 070 ₸ 100 µl 341 070 ₸

Order now and get it on Thursday February 25, 2021

HRP Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192080)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
HRP Rabbit monoclonal [EPR1473] to CDKN2A/p16INK4a
Suitable for: WB, IHC-P
Reacts with: Human
Conjugation: HRP

Product name

HRP Anti-CDKN2A/p16INK4a antibody [EPR1473]
See all CDKN2A/p16INK4a primary antibodies
Description

HRP Rabbit monoclonal [EPR1473] to CDKN2A/p16INK4a
Host species

Rabbit
Conjugation

HRP
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HEK293. IHC. Human lung - Squamous cell carcinoma tissue
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR1473
Isotype

IgG
Research areas

Western blot - HRP Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192080)

All lanes : HRP Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192080) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 17 kDa
Observed band size: 17 kDa

Exposure time: 20 minutes

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab192080 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192080)

IHC image of CDKN2A/p16INK4a staining in a section of formalin-fixed paraffin-embedded Human lung squamous cell carcinoma. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 mins. The section was incubated with ab192080 at a working dilution of 1 in 100 overnight at +4°C. The section was counterstained with haematoxylin and mounted with DPX.
This image shows an area of stained macrophage cells. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192080)

IHC image of CDKN2A/p16INK4a staining in a section of formalin-fixed paraffin-embedded Human lung squamous cell carcinoma. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 mins. The section was incubated with ab192080 at a working diltuion of 1 in 100 overnight at +4°C. The section was counterstained with haematoxylin and mounted with DPX.
This image shows an area of cancerous tissue. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
HRP Anti-CDKN2A/p16INK4a antibody [EPR1473] (ab192080)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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