Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3218] to Adiponectin - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Adiponectin antibody [EPR3218] - BSA and Azide free
See all Adiponectin primary antibodies -
Description
Rabbit monoclonal [EPR3218] to Adiponectin - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fat, placenta and plasma lysates. HUH7, Human adipose and plasma lysate. Mouse white adipose and plasma lysate. Rat white adipose and plasma lysate. Rat-1 whole cell lysate
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General notes
ab226122 is the carrier-free version of ab133347 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab226122 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3218 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution (Purified)
Lane 1 : Mouse white adipose lysate
Lane 2 : Mouse plasma lysate
Lane 3 : Rat-1 (Rat embryonic fibroblast) whole cell lysate
Lane 4 : Rat white adipose lysate
Lane 5 : Rat plasma lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?This data was developed using ab133347, the same antibody clone in a different buffer formulation.
The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID: 15855314, 23967179).
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All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution (Purified)
Lane 1 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : Human adipose lysate
Lane 3 : Human plasma lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?This data was developed using ab133347, the same antibody clone in a different buffer formulation.
The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID: 15855314, 23967179).
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All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution
Lane 1 : Human fat lysate
Lane 2 : Human placenta lysate
Lane 3 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDaThis data was developed using ab133347, the same antibody clone in a different buffer formulation.
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All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347)
Lane 1 : Human plasma lysate treated with Beta-ME and heat
Lane 2 : Human plasma lysate treated with heat only
Lane 3 : Human plasma lysate treated with Beta-ME only
Lane 4 : Human plasma lysate untreated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDaThis data was developed using ab133347, the same antibody clone in a different buffer formulation.
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