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Cardiovascular Lipids / Lipoproteins Adipose Related Acrp

Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3218] to Adiponectin - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Adiponectin antibody [EPR3218] - BSA and Azide free
    See all Adiponectin primary antibodies
  • Description

    Rabbit monoclonal [EPR3218] to Adiponectin - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fat, placenta and plasma lysates. HUH7, Human adipose and plasma lysate. Mouse white adipose and plasma lysate. Rat white adipose and plasma lysate. Rat-1 whole cell lysate
  • General notes

    ab226122 is the carrier-free version of ab133347 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab226122 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3218
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Adipose Related
    • Acrp
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Neurology process
    • Metabolism
    • Stem Cells
    • Mesenchymal Stem Cells
    • Adipogenesis
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Heart disease
    • Metabolism
    • Types of disease
    • Metabolic disorders

Images

  • Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)
    Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)
    All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution (Purified)

    Lane 1 : Mouse white adipose lysate
    Lane 2 : Mouse plasma lysate
    Lane 3 : Rat-1 (Rat embryonic fibroblast) whole cell lysate
    Lane 4 : Rat white adipose lysate
    Lane 5 : Rat plasma lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 26 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab133347, the same antibody clone in a different buffer formulation.

    The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID: 15855314, 23967179).

  • Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)
    Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)
    All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution (Purified)

    Lane 1 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : Human adipose lysate
    Lane 3 : Human plasma lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 26 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?



    This data was developed using ab133347, the same antibody clone in a different buffer formulation.

    The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID: 15855314, 23967179).

  • Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)
    Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)
    All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution

    Lane 1 : Human fat lysate
    Lane 2 : Human placenta lysate
    Lane 3 : Human plasma lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 26 kDa



    This data was developed using ab133347, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)
    Western blot - Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)
    All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347)

    Lane 1 : Human plasma lysate treated with Beta-ME and heat
    Lane 2 : Human plasma lysate treated with heat only
    Lane 3 : Human plasma lysate treated with Beta-ME only
    Lane 4 : Human plasma lysate untreated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 26 kDa



    This data was developed using ab133347, the same antibody clone in a different buffer formulation.

     

  • Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)
    Anti-Adiponectin antibody [EPR3218] - BSA and Azide free (ab226122)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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