Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6626] to ADIPOR1 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free
    See all ADIPOR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6626] to ADIPOR1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab240022 is the carrier-free version of ab126611 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab240022 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Adiponectin Receptor 1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
    Immunocytochemistry/ Immunofluorescence - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)

    Immunocytochemistry of HeLa cells fixed in 4% PFA with purified ab126611 at a dilution of 1/400. An Alexa Fluor® goat anti-rabbit antibody was used as the secondary and the sample was counter stained wtih DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126611).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)

    Immunohistochemical staining of paraffin embedded human endometrium with purified ab126611 at a dilution of 1/200. The seconday was a HRP polymer for rabbit IgG amd the counter stain used was hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126611).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)

    Immunohistochemical staining of ADIPOR1 in formalin-fixed, paraffin-embedded human kidney tissue by unpurified ab126611, at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126611).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
    Flow Cytometry - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)

    Overlay histogram showing HepG2 cells stained with unpurified ab126611 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126611, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126611).

  • OI-RD Scanning - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
    OI-RD Scanning - Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)

    Equilibrium disassociation constant (KD) with unpurified ab126611.
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126611).

  • Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)
    Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-ADIPOR1 antibody [EPR6626] - BSA and Azide free (ab240022)

© 2021 Astana Biomed Group LLP. All rights reserved.