Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3949] to Tcl1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC, WB, ICC/IF, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Tcl1 antibody [EPR3949] - BSA and Azide free
    See all Tcl1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3949] to Tcl1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC, WB, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab199188 is the carrier-free version of ab108978.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)

    ab108978 staining Tcl1 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/700. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

    Negative control 1: PBS in place of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

  • Flow Cytometry - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)
    Flow Cytometry - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)

    ab108978 staining Tcl1 in the human cell line Ramos (human Burkitt's lymphoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/400. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

  • Immunocytochemistry/ Immunofluorescence - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)
    Immunocytochemistry/ Immunofluorescence - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)

    ab108978 staining Tcl1 in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab108978 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

  • Flow Cytometry - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)
    Flow Cytometry - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)

    Overlay histogram showing Ramos cells stained with ab108978 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108978, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab108978.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)

    ab108978 showing negative staining in Skeletal muscle tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)

    ab108978 showing positive staining in Normal spleen tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)

    ab108978 showing negative staining in Normal brain tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)

    ab108978 showing negative staining in Normal kidney tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)

    ab108978 showing negative staining in Normal stomach tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108978).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)
    Anti-Tcl1 antibody [EPR3949] - BSA and Azide free (ab199188)

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