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Cardiovascular Lipids / Lipoproteins Adipose Related Acrp

Anti-Adiponectin antibody [EPR3218] (ab133347)

Price and availability

298 185 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-Adiponectin antibody [EPR3218] (ab133347)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3218] to Adiponectin
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Adiponectin antibody [EPR3218]
    See all Adiponectin primary antibodies
  • Description

    Rabbit monoclonal [EPR3218] to Adiponectin
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to residues in Human Adiponectin (UniProt Q15848).

  • Positive control

    • WB: Human fat, placenta and plasma lysates. HUH7, Human adipose and plasma lysate. Mouse white adipose and plasma lysate. Rat white adipose and plasma lysate. Rat-1 whole cell lysate
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3218
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Adipose Related
    • Acrp
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Neurology process
    • Metabolism
    • Stem Cells
    • Mesenchymal Stem Cells
    • Adipogenesis
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Heart disease
    • Metabolism
    • Types of disease
    • Metabolic disorders

Images

  • Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
    Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
    All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution (Purified)

    Lane 1 : Mouse white adipose lysate
    Lane 2 : Mouse plasma lysate
    Lane 3 : Rat-1 (Rat embryonic fibroblast) whole cell lysate
    Lane 4 : Rat white adipose lysate
    Lane 5 : Rat plasma lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 26 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID: 15855314, 23967179).

  • Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
    Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
    All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution (Purified)

    Lane 1 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : Human adipose lysate
    Lane 3 : Human plasma lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 26 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?



    The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID: 15855314, 23967179).

  • Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
    Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
    All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution

    Lane 1 : Human fat lysate
    Lane 2 : Human placenta lysate
    Lane 3 : Human plasma lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 26 kDa

  • Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
    Western blot - Anti-Adiponectin antibody [EPR3218] (ab133347)
    All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347)

    Lane 1 : Human plasma lysate treated with Beta-ME and heat
    Lane 2 : Human plasma lysate treated with heat only
    Lane 3 : Human plasma lysate treated with Beta-ME only
    Lane 4 : Human plasma lysate untreated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 26 kDa

  • Anti-Adiponectin antibody [EPR3218] (ab133347)
    Anti-Adiponectin antibody [EPR3218] (ab133347)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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