Mouse Adiponectin ELISA Kit (ab226900)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 26 pg/ml
- Range: 78.1 pg/ml - 5000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Adiponectin ELISA Kit
See all Adiponectin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 3.7% Inter-assay Sample n Mean SD CV% Serum 3 3.1% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
26 pg/ml -
Range
78.1 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 108 106% - 109% Cell culture media 102 99% - 106% Hep Plasma 112 109% - 114% EDTA Plasma 108 102% - 116% Cit plasma 99 93% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse Adiponectin ELISA Kit (ab226900) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Adiponectin protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Adiponectin with 26 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Adiponectin is a 30kDa monomer that combines to form trimers (low molecular weight), hexamers (middle molecular weight), and other higher molecular weight multimers. Adiponectin is an important adipokine involved in fat metabolism and insulin sensitivity. Insulin stimulates the secretion of adiponectin. Decreased levels of adiponectin are associated with insulin resistance, whereas high levels of adiponectin are associated with reduced risk of type-2 diabetes.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml 10X Mouse Adiponectin Capture Antibody 1 x 600µl 10X Mouse Adiponectin Detector Antibody 1 x 600µl Mouse Adiponectin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. -
Tissue specificity
Synthesized exclusively by adipocytes and secreted into plasma. -
Involvement in disease
Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. -
Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
Domain
The C1q domain is commonly called the globular domain. -
Post-translational
modificationsHydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- 30 kDa adipocyte complement related protein
- 30 kDa adipocyte complement-related protein
- ACDC
see all -
Database links
- Entrez Gene: 11450 Mouse
- SwissProt: Q60994 Mouse
- Unigene: 3969 Mouse
Images
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Other - Mouse Adiponectin ELISA Kit (ab226900)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse Adiponectin Competitor standard curve comparisonStandard curve comparison between Mouse Adiponectin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of Mouse Adiponectin standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Adiponectin in Mouse serum, and plasma samplesThe concentrations of Adiponectin were measured in duplicates, interpolated from the Adiponectin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:6667, plasma (EDTA) 1:6667, plasma (heparin) 1:6667, and plasma (citrate) 1:6667. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Adiponectin concentration was determined to be 2212 ng/mL in serum, 2168 ng/mL in plasma (EDTA), 2129 ng/mL in plasma (heparin) and 1845 ng/mL in plasma (citrate).
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Interpolated concentrations of native Adiponectin in Mouse cell culture supernatant samplesThe concentrations of Adiponectin were measured in duplicates, interpolated from the Adiponectin standard curves and corrected for sample dilution. Undiluted samples are as follows: lung supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Adiponectin concentration was determined to be 386 pg/mL in lung supernatant.
