Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (ab273068)
Key features and details
- Expression system: CHO cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Indirect ELISA, SDS-PAGE, Functional Studies
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Product name
Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active)
See all SARS-CoV-2 Spike Glycoprotein S1 proteins and peptides -
Biological activity
Flow cytometry assay showing ab273068 can bind to ACE2 overexpressing cells. ACE2 overexpressing cells were stained with ab273068, followed by an anti-spike protein antibody, and a fluorescence-conjugated secondary antibody.
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Purity
> 95 % SDS-PAGE. -
Expression system
CHO cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human coronavirus -
Predicted molecular weight
76 kDa -
Actual molecular weight
125 kDa -
Amino acids
16 to 685 -
Tags
His tag C-Terminus
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Images
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Indirect ELISA - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (ab273068)
Indirect ELISA showing primary antibody ab273073 (CR3022, human chimeric) binding to the antigens ab272105 (recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (sheep Fc fusion)) and ab273068 (recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active)). Plates were coated with 100ng/well ab272105 or ab273068 and binding of ab273073 assessed in serial dilution from 200ng/ml primary antibody in duplicate. Binding was detected using ab98624, an anti-human Fc secondary conjugated to HRP. Data are represented as the mean and error bars represent standard deviation.
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Indirect ELISA - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (ab273068)Indirect ELISA showing primary antibody ab273074 (CR3022, rabbit chimeric) binding to the antigen ab273068 (recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active)). Plates were coated with 100ng/well ab272105 or ab273068 and binding of ab273073 assessed in serial dilution from 200ng/ml primary antibody in duplicate. Binding was detected using ab97080, an anti-rabbit H&L secondary conjugated to HRP. Data are represented as the mean and error bars represent standard deviation.
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Functional Studies - Recombinant SARS Coronavirus Spike Glycoprotein Mosaic (S1) (Active) (ab273068)Flow cytometry assay showing ab273068 can bind to ACE2 overexpressing cells. ACE2 overexpressing cells were stained with ab273068, followed by an anti-spike protein antibody, and a fluorescence-conjugated secondary antibody.
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SDS-PAGE - Recombinant SARS Coronavirus Spike Glycoprotein Mosaic (S1) (Active) (ab273068)SDS-PAGE analysis of ab273068.