COVID-19 N-Protein ELISA Kit (ab274341)
Key features and details
- Sensitivity: 0.07 ng/ml
- Range: 0.069 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
Overview
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Product name
COVID-19 N-Protein ELISA Kit
See all COVID-19 N-Protein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum -
Assay type
Quantitative -
Sensitivity
0.07 ng/ml -
Range
0.069 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 97.9 83% - 111% Cell culture media 87.26 83% - 92% -
Assay duration
Multiple steps standard assay -
Product overview
COVID-19 N-Protein ELISA Kit (ab274341) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of COVID-19 N Protein in serum (plasma is not recommended in this assay) and cell culture supernatants.
This assay employs an antibody specific for COVID-19 N Protein coated on a 96-well plate. Standards and samples are pipetted into the wells and COVID-19 N Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-COVID-19 N Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COVID-19 N Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Antibody 2 vials 100X HRP-Strepavidin Concentrate 1 x 200µl Lyophilized Standard Protein 2 vials Microplate 1 unit Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
Images
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COVID-19 N Protein Standard Curve
This standard curve is for demonstration only. A standard curve must be run with each assay.