Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA and Azide free (ab281299)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HL455] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free
- Suitable for: WB, ICC, Sandwich ELISA, Indirect ELISA, IHC-P
Overview
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Product name
Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA and Azide free
See all SARS-CoV-2 nucleocapsid protein primary antibodies -
Description
Rabbit monoclonal [HL455] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, Sandwich ELISA, Indirect ELISA, IHC-Pmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Recombinant full length protein corresponding to Human coronavirus SARS-CoV-2 nucleocapsid protein. Strain Wuhan-Hu-1.
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Positive control
- IHC-P: HEK-293T (transfected with -SARS-CoV-2 nucleocapsid protein) cell pellet block. ICC: HEK-293T cells transfected with -SARS-CoV-2 nucleocapsid . WB: VeroE6 and HEK-293T transfected with SARS-CoV-2 nucleocapsid, whole cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HL455 -
Isotype
IgG -
Research areas
Images
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Mock and SARS-CoV-2 nucleocapsid transfected HEK-293T cell FFPE Cell Pellet Block. SARS-CoV-2 nucleocapsid (green) was labelled using ab281299 at 1/1000 dilution immunohistochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval achieved with Citrate buffer at pH 6.0 for 15 minutes.
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All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA and Azide free (ab281299) at 1/5000 dilution
Lane 1 : 293T whole cell extracts (30 µg) non-transfected
Lane 2 : 293T whole cell extracts (30 µg) transfected with SARS-CoV NP
Lane 3 : 293T whole cell extracts (30 µg) transfected with SARS-CoV-2 (COVID-19) NP
Lane 4 : 293T whole cell extracts (30 µg) transfected with MERS-CoV NP
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibodyUpper panel probed with ab281299.
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Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (1000-15.63 ng/mL). Coated protein was probed with ab281299 at 1 µg/mL. HRP-Rabbit IgG antibody at 1/10000 dilution was used to detect bound primary antibody.
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All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA and Azide free (ab281299) at 1/5000 dilution
Lane 1 : Vero E6 whole cell extracts (30 µg) non-transfected
Lane 2 : Vero E6 whole cell extracts (30 µg) transfected with SARS-CoV-2 (COVID-19) nucleocapsid
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
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Mock and SARS-CoV-2 nucleocapsid transfected HEK-293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 nucleocapsid (green) was labeled using ab281299 at 1/1000 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue).
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All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA and Azide free (ab281299) at 1/5000 dilution
Lane 1 : 293T whole cell extracts (30 µg) non-transfected
Lane 2 : 293T whole cell extracts (30 µg) transfected with SARS-CoV-2 (COVID-19) nucleocapsid
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
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Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281301 as capture antibody at concentration of 5 µ/mL and HRP-conjugated ab281299 as detection antibody at concentration of 1 µg/mL.
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Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (50 ng). Coated protein probed with ab281299 (4.8*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (1:10000) detected bound primary antibody.
EC50 : 0.006 nM