Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA and Azide free (ab281299)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [HL455] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free
  • Suitable for: WB, ICC, Sandwich ELISA, Indirect ELISA, IHC-P

Overview

  • Product name

    Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA and Azide free
    See all SARS-CoV-2 nucleocapsid protein primary antibodies

  • Description

    Rabbit monoclonal [HL455] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, Sandwich ELISA, Indirect ELISA, IHC-Pmore details

  • Species reactivity

    Reacts with: SARS-CoV-2

  • Immunogen

    Recombinant full length protein corresponding to Human coronavirus SARS-CoV-2 nucleocapsid protein. Strain Wuhan-Hu-1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: HEK-293T (transfected with -SARS-CoV-2 nucleocapsid protein) cell pellet block. ICC: HEK-293T cells transfected with -SARS-CoV-2 nucleocapsid . WB: VeroE6 and HEK-293T transfected with SARS-CoV-2 nucleocapsid, whole cell extract.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA free (ab281299)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA free (ab281299)

    Mock and SARS-CoV-2 nucleocapsid transfected HEK-293T cell FFPE Cell Pellet Block. SARS-CoV-2 nucleocapsid (green) was labelled using ab281299 at 1/1000 dilution immunohistochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval achieved with Citrate buffer at pH 6.0 for 15 minutes.

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA free (ab281299)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA free (ab281299)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA and Azide free (ab281299) at 1/5000 dilution

    Lane 1 : 293T whole cell extracts (30 µg) non-transfected
    Lane 2 : 293T whole cell extracts (30 µg) transfected with SARS-CoV NP
    Lane 3 : 293T whole cell extracts (30 µg) transfected with SARS-CoV-2 (COVID-19) NP
    Lane 4 : 293T whole cell extracts (30 µg) transfected with MERS-CoV NP

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    Upper panel probed with ab281299.

  • Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA free (ab281299)
    Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA free (ab281299)

    Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (1000-15.63 ng/mL). Coated protein was probed with ab281299 at 1 µg/mL. HRP-Rabbit IgG antibody at 1/10000 dilution was used to detect bound primary antibody.

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA free (ab281299)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA free (ab281299)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA and Azide free (ab281299) at 1/5000 dilution

    Lane 1 : Vero E6 whole cell extracts (30 µg) non-transfected
    Lane 2 : Vero E6 whole cell extracts (30 µg) transfected with SARS-CoV-2 (COVID-19) nucleocapsid

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody
  • Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA free (ab281299)
    Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA free (ab281299)

    Mock and SARS-CoV-2 nucleocapsid transfected HEK-293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 nucleocapsid (green) was labeled using ab281299 at 1/1000 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue).

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA free (ab281299)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA free (ab281299)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA and Azide free (ab281299) at 1/5000 dilution

    Lane 1 : 293T whole cell extracts (30 µg) non-transfected
    Lane 2 : 293T whole cell extracts (30 µg) transfected with SARS-CoV-2 (COVID-19) nucleocapsid

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody
  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA free (ab281299)
    Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA free (ab281299)

    Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281301 as capture antibody at concentration of 5 µ/mL and HRP-conjugated ab281299 as detection antibody at concentration of 1 µg/mL.

  • Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA free (ab281299)
    Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455] - BSA free (ab281299)

    Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (50 ng). Coated protein probed with ab281299 (4.8*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (1:10000) detected bound primary antibody.
    EC50 : 0.006 nM

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