SARS-CoV-2 IgM ELISA Kit (ab275299)
Key features and details
- Sample type: Cit plasma, EDTA Plasma, Lithium Heparin Plasma, Serum
- Detection method: Colorimetric
- Assay type: Semi-quantitative
Overview
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Product name
SARS-CoV-2 IgM ELISA Kit
See all SARS-CoV-2 Spike Glycoprotein S1 kits -
Detection method
Colorimetric -
Sample type
Serum, EDTA Plasma, Cit plasma, Lithium Heparin Plasma -
Assay type
Semi-quantitative -
Assay duration
Multiple steps standard assay -
Product overview
SARS-CoV-2 IgM ELISA Kit (ab275299) is an Enzyme-Linked Immunosorbent Assay (ELISA) intended for semi-quantitative detection of IgM antibodies to SARS-CoV-2 in human serum or plasma collected in potassium EDTA, sodium citrate or lithium heparin.
This kit is intended for use as an aid in identifying individuals with an adaptive immune response to SARS-CoV-2, indicating recent or prior infection. Understanding the timing, duration and effectiveness of humoral immune responses in individuals previously infected with SARS-CoV-2 will be important for conducting vaccine and epidemiological research. At this time, it is unknown for how long antibodies persist following infection and if the presence of antibodies confers protective immunity.
Results are for the detection of SARS-CoV-2 antibodies. IgM antibodies to SARS-CoV-2 are generally detectable in blood several days after initial infection, although the duration of time antibodies are present post-infection is not well characterized. Individuals may have detectable virus present for several weeks following seroconversion.
The sensitivity of SARS-CoV-2 IgM ELISA early after infection is unknown. Negative results do not preclude acute SARS-CoV-2 infection.
False positive results for SARS-CoV-2 IgM ELISA may occur due to cross-reactivity from pre-existing antibodies to SARS-CoV 1 or other possible causes. -
Notes
This kit provides for an indirect ELISA, in which a recombinant receptor binding domain (RBD) of the Spike1 protein of SARS-CoV 2 is coated on the wells of the microtiter plate. Antibodies to SARS-CoV-2 RBD when present in the test sample bind specifically to the RBD protein. After sample binding, unbound proteins and molecules are washed off, and a HRP-conjugated detection antibody is added to the wells to bind to the captured anti-SARS-CoV2 IgM antibodies. The chromogenic substrate TMB (3,3’,5,5’tetramethylbenzidine) is then added. This reaction produces a blue product, which turns yellow when the reaction is terminated by addition of dilute sulfuric acid. The absorbance of the yellow product at 450 nm, corrected for plate imperfections by subtracting the absorbance at 570 nm, is proportional to the amount of RBD-specific anti-SARS-CoV-2 IgM present in the sample.
After determining that the values for the Positive Control and Negative Control are valid and acceptable by comparing them to the value for the Calibrator, values for samples are compared to the Calibrator to generate a ratio. Ratios above a cutoff indicate positive samples and values below a cutoff indicate negative samples.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 96 well Precoated SARS-CoV-2 S1-RBD plate 1 unit Assay Dilution Buffer (ready to use) 1 vial Calibrator: Recombinant Human anti-RBD IgM antibody (ready to use) 1 vial Detection Antibody: Goat Anti-Human IgM-Fc antibody – HRP conjugated (ready to use) 1 vial Negative Control: Dilute normal human serum (ready to use) 1 vial One Component TMB substrate: TMB/Hydrogen Peroxide (ready to use) 1 vial Positive Control: Recombinant Human anti-RBD IgM (ready to use) 1 vial Sealing Tape 3 units Stop solution (0.18 M Sulfuric Acid; ready to use) 1 vial TNT Wash Buffer packet for reconstitution 1 vial -
Research areas
