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Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [HL249] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free
  • Suitable for: IHC-P, ELISA, WB, ICC, Sandwich ELISA

Overview

  • Product name

    Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free
    See all SARS-CoV-2 nucleocapsid protein primary antibodies

  • Description

    Rabbit monoclonal [HL249] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ELISA, WB, ICC, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: SARS-CoV-2

  • Immunogen

    Recombinant full length protein corresponding to Human coronavirus SARS-CoV-2 nucleocapsid protein. Strain Wuhan-Hu-1

  • Positive control

    • ICC: SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T cells. IHC-P: SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T cells. WB: SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T cells. ELISA: Rrecombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein.

Properties

Images

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295) at 1/5000 dilution

    Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : SARS-CoV NP transfected 293T whole cell extracts
    Lane 3 : SARS-CoV-2 (COVID-19) NP transfected 293T whole cell extracts
    Lane 4 : MERS-CoV transfected 293T whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295)

    Mock and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) nucleocapsid stained by ab281295 (green) at 1/1000 dilution in a immunohistochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 15 minutes.

  • Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295)
    Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295)

    Mock and transfected SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) nucleocapsid stained by ab281295 (green) at 1/1000 dilution in immunocytochemistry analysis. Fluoroshield with DAPI (blue).

  • Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295)
    Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295)

    Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (50 ng). Coated protein probed with ab281295 (2.4*10⁻⁶ ~ 20 nM). HRP-conjugated Rabbit IgG antibody (1:10000) detected bound primary antibody.
    EC50: 0.016 nM

  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295)
    Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295)

    Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281302 as capture antibody at concentration of 5 µg/mL and HRP-conjugated ab281295 as detection antibody at concentration of 1 µg/mL.

    Please notice that ab281295 needs to be conjugated to HRP to function as the detection antibody when paired to ab281302.

  • Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295)
    Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295)

    Indirect ELISA analysis was performed by coating plate with 50 µL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (A and B) at concentrations ranging from 0.015 µg/mL to 1 µg/mL. The coated protein is detected with ab281295 at 1 µg/mL. HRP-conjugated Rabbit IgG antibody was diluted at 1/10000 and used to detect the primary antibody.

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL249] - BSA and Azide free (ab281295) at 1/5000 dilution

    Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : SARS-CoV-2 (COVID-19) NP transfected 293T whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    Lane 1 : HRP-conjugated anti-rabbit IgG
    Lane 2 : HRP-conjugated anti-rabbit IgG antibody

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