Recombinant Human coronavirus SARS E2 protein (Tagged) (ab270834)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: GST tag C-Terminus
- Suitable for: SDS-PAGE, ELISA, WB
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Product name
Recombinant Human coronavirus SARS E2 protein (Tagged) -
Purity
> 95 % SDS-PAGE.
GS-4B Sepharose-Affinity Purification. -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human coronavirus -
Predicted molecular weight
35 kDa including tags -
Molecular weight information
May appear as multiple bands on SDS-PAGE (intact protein at 34.7 kDa, GST fusion protein at 26 kDa and the E fragment at 8.7 kDa) -
Amino acids
1 to 76 -
Tags
GST tag C-Terminus -
Additional sequence information
Derived from CUHK-L2. Contains the E protein immunodominant region. Not full-length E protein. Contains GST fusion partner. Immunoreactive with SARS positive sera.
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Preparation and Storage
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Relevance
SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The complete sequence of the SARS virus release the coronavirus contains 25 open reading frames. SARS E2 glycoprotein precursor is a 139kDa glycoprotein. It contains a superantigen between residues 690 through 1050 which has relationship to T-cell Receptor alpha-beta V chain protein.
