Recombinant Human coronavirus SARS-COV-2 Nsp1 protein (His tag) (ab277123)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant Human coronavirus SARS-COV-2 Nsp1 protein (His tag) -
Purity
> 90 % SDS-PAGE. -
Endotoxin level
Expression system
Escherichia coliAccession
Protein length
Protein fragmentAnimal free
NoNature
Recombinant-
Species
Human coronavirus -
Sequence
MESLVPGFNE KTHVQLSLPV LQVRDVLVRG FGDSVEEVLS EARQHLKDGT CGLVEVEKGV LPQLEQPYVF IKRSDARTAP HGHVMVELVA ELEGIQYGRS GETLGVLVPH VGEIPVAYRK VLLRKNGNKG AGGHSYGADL KSFDLGDELG TDPYEDFQEN WNTKHSSGVT RELMRELNGG -
Predicted molecular weight
22 kDa including tags -
Molecular weight information
The protein has a calculated MW of 21.7 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) -
Amino acids
1 to 180 -
Tags
His tag C-Terminus -
Additional sequence information
10X His tag. YP_009725297.1
Associated products
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Related Buffer
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab277123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Additional notes
To avoid surface adsorption, loss and inactivation, the reconstituted protein must NOT be aliquoted to less than 10 ug per vial.
Under the following conditions carrier protein (0.1% w/v ) HSA or BSA MUST be included in the reconstitution buffer:
1. Aliquots of
2. The concentration is less than the recommended 200 ug/ml
3. Long term storage
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 90% PBS, 10% Trehalose
0.2 µm filtered solution -
ReconstitutionReconstitute with 500 µL sterile deionized water to a stock solution of 200 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing. Stable at -70°C for 3 months under sterile conditions after reconstitution.
Images
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SDS-PAGE - Recombinant SARS Nsp1 protein (ab277123)
SDS-PAGE of ab277123.
The protein has a calculated MW of 21.7 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab277123 has not yet been referenced specifically in any publications.
Preparation and Storage
-
Applications
SDS-PAGE
-
Form
Lyophilized -
Additional notes
To avoid surface adsorption, loss and inactivation, the reconstituted protein must NOT be aliquoted to less than 10 ug per vial.
Under the following conditions carrier protein (0.1% w/v ) HSA or BSA MUST be included in the reconstitution buffer:
1. Aliquots of
2. The concentration is less than the recommended 200 ug/ml
3. Long term storage
-
Concentration information loading...
Images
-
SDS-PAGE - Recombinant SARS Nsp1 protein (ab277123)
SDS-PAGE of ab277123.
The protein has a calculated MW of 21.7 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE).