Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [HL6] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free
  • Suitable for: WB, ICC, IHC-P, Flow Cyt, Indirect ELISA, Sandwich ELISA

Overview

  • Product name

    Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free
    See all SARS-CoV-2 Spike Glycoprotein S1 primary antibodies

  • Description

    Rabbit monoclonal [HL6] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, IHC-P, Flow Cyt, Indirect ELISA, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: SARS-CoV-2

  • Immunogen

    Recombinant fragment within Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. SARS-CoV-2 (strain Wuhan-Hu-1)

  • Positive control

    • IHC-P: SARS-CoV-2 (COVID-19) infected human placenta an human lung tissue. Flow cyt: SARS-CoV-2 (2019-nCoV) Spike 293T cells. WB: SARS-CoV-2 Spike transfected 293T cells. ICC: SARS-CoV-2 Spike transfected 293T cells. ELISA: Recombinant full-length SARS-CoV-2 spike protein, SARS-CoV-2 spike (full length) transfected 293T whole cell extracts.

Properties

Images

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311) at 1/5000 dilution

    Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : SARS-CoV Spike transfected 293T whole cell extrac
    Lane 3 : SARS-CoV-2 (COVID-19) Spike transfected 293T whole cell extract
    Lane 4 : MERS Spike transfected 293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    5% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281311)

    Paraffin embedded SARS-CoV-2 (COVID-19) infected human placenta (left) and normal human placenta (right) tissue stained for SARS-CoV-2 (COVID-19) nucleocapsid protein using ab281311 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281311)

    Paraffin embedded SARS-CoV-2 (COVID-19) infected human lung tissue stained for SARS-CoV-2 (COVID-19) nucleocapsid protein using ab281311 in immunohistochemical analysis.

  • Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)

    Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with ab281311 (2.4*10⁻⁶ ~ 20 nM). HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
    EC50: 0.251 nM

  • Sandwich ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    Sandwich ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)

    Sandwich ELISA detection of non-transfected and SARS-CoV-2 spike (full length) transfected 293T whole cell extracts using 5 µg/mL of capture antibody and ab281311 as detection antibody at 1 µg/mL. HRP-conjugated Rabbit IgG antibody at 1/10000 dilution was used to detect the primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281311)

    Mock and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) Spike S1 stained by ab281311 at 1/1000 dilution in immunohistochemical analysis. Fluoroshield with DAPI (blue).

    Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 15 minutes.

  • Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    Mock and SARS-CoV-2 Spike-transfected 293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) spike stained (green) by ab281311 at 1/1000 dilution in Immunocytochemical analysis. Fluoroshield with DAPI (blue).
  • Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)

    Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein or recombinant SARS-CoV spike S1 subunit protein (62.5-4000 ng/mL). Coated protein probed with ab281311 at 1 µg/mL. HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.

  • Flow Cytometry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    Flow Cytometry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)

    ab281311 detects SARS-CoV-2 (COVID-19) spike protein by flow cytometry analysis.  SARS-CoV-2 (2019-nCoV) Spike 293T cells. Unlabelled sample was used as a control (blue) and ab281311 at 1/50 dilution (red). Acquisition of 20,000 events were collected for flow cytometry analysis.

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311) at 1/5000 dilution

    Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : SARS-CoV-2 (COVID-19) Spike transfected 293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    5% SDS-PAGE

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