Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)
![Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)](https://www.abcam.com/ps/products/281/ab281294/Images/ab281294-456632-SARSCoV2-nucleocapsid-protein-antibody-HL146-wb.jpg "Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HL146] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free
- Suitable for: ELISA, WB, ICC, Sandwich ELISA
Overview
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Product name
Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free
See all SARS-CoV-2 nucleocapsid protein primary antibodies -
Description
Rabbit monoclonal [HL146] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, ICC, Sandwich ELISAmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Recombinant full length protein corresponding to Human coronavirus SARS-CoV-2 nucleocapsid protein. Strain Wuhan-Hu-1
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Positive control
- ICC: SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T cells. WB: SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T cells. ELISA: Recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HL146 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294) at 1/5000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : SARS-CoV NP transfected 293T whole cell extracts
Lane 3 : SARS-CoV-2 (COVID-19) NP transfected 293T whole cell extracts
Lane 4 : MERS-CoV NP transfected 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
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![Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)](https://www.abcam.com/ps/products/281/ab281294/Images/ab281294-4-SARSCoV2-covid19-nucleocapsid-antibody-HL146-immunocytomechemistry-hek293t.jpg)
Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)Mock and SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) nucleocapsid was stained by ab281294 (green) at 1/1000 dilution by immunofluorescent analysis. Fluoroshield with DAPI (blue).
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![Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)](https://www.abcam.com/ps/products/281/ab281294/Images/ab281294-3-SARSCoV2-covid19-nucleocapsid-antibody-HL146-indirectelisa.jpg)
Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein at 50ng. Coated protein probed with ab281294 at 2.4*10-6 ~ 20 nM. HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody. EC50: 0.05 nM
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![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)](https://www.abcam.com/ps/products/281/ab281294/Images/ab281294-2-SARSCoV2-covid19-nucleocapsid-antibody-HL146-westernblot-hek93T-cells.jpg)
Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294) at 1/5000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : SARS-CoV-2 (COVID-19) NP transfected 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
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![Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)](https://www.abcam.com/ps/products/281/ab281294/Images/ab281294-1-SARSCoV2-covid19-nucleocapsid-antibody-HL146-sandwichelisa.jpg)
Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281294 as capture antibody at concentration of 5 µg/ml and HRP-conjugated ab281299 as detection antibody at concentration of 1 µg/ml.