COVID-19 N-Protein Human IgM ELISA Kit (ab276184)
Key features and details
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Semi-quantitative
Overview
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Product name
COVID-19 N-Protein Human IgM ELISA Kit
See all COVID-19 N-Protein kits -
Detection method
Colorimetric -
Sample type
Serum, Plasma -
Assay type
Semi-quantitative -
Assay duration
Multiple steps standard assay -
Product overview
COVID-19 N-Protein Human IgM ELISA Kit (ab276184) is an in vitro indirect ELISA for the semi-quantitative measurement of human IgM antibody against SARS-CoV-2 N protein in human serum.
Standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARSCoV-2 N protein, which combines with the corresponding antibody present in a sample. When a secondary anti-human Antibody-HRP is added, a complex of Antibody-HRP human IgM antibody virus N antigen forms on the microplate. A TMB substrate is added and a blue color is generated. The depth of color is relative to the amount of the anti-SARS-CoV-2 IgM antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The Positive Control is from an inactivated serum sample which contains SARS-COV-2 N protein human IgM antibody. We do not know the exact amount of SARS-COV-2 N protein human IgM antibody in the Positive Control sample. The Positive Control can be used as calibration curve for interpretation purposes in different assays.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml Assay Diluent A 1 x 30ml HRP-anti Human IgM Antibody 1 vial HRP-anti positive control IgM Antibody 1 vial Negative Control 1 vial Positive Control 1 vial SARS-CoV-2 N protein coated Microplate 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
