Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [HL257] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free
  • Suitable for: Indirect ELISA, IHC-P, WB, ICC

Overview

  • Product name

    Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free
    See all SARS-CoV-2 Spike Glycoprotein S1 primary antibodies

  • Description

    Rabbit monoclonal [HL257] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Indirect ELISA, IHC-P, WB, ICCmore details

  • Species reactivity

    Reacts with: SARS-CoV-2

  • Immunogen

    Synthetic peptide within Human coronavirus SARS-CoV-2 Spike Glycoprotein S1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Strain Wuhan-Hu-1.

  • Positive control

    • IHC-P: SARS-CoV-2 (COVID-19) infected mouse lung, human lung and placenta tissue. ICC: SARS-CoV-2 (COVID-19) Spike transfected HEK-293T cells. WB: SARS-CoV-2 (COVID-19) Spike transfected HEK-293T whole cell extracts, SARS-CoV-2 (COVID-19) Spike RBD protein. ELISA: Recombinant SARS-CoV-2 (COVID-19) Spike RBD protein.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)
    Paraffin-embedded non-infected (left) and infected (right) human placenta tissue stained for SARS-CoV-2 (COVID-19) Spike RBD using ab281306 at 1/500 dilution in immunohistochemical analysis. Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 20 minutes.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)
    Paraffin-embedded non-infected (left) and infected (right) human lung tissue stained for SARS-CoV-2 (COVID-19) Spike RBD using ab281306 at 1/1000 dilution in immunohistochemical analysis. Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 20 minutes.
  • Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)
    Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)

    Mock and SARS-CoV-2 (COVID-19) Spike transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) Spike RBD stained by ab281306 (green) at 1/1000 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval achieved with Citrate buffer at pH 6.0 for 15 minutes.

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)
    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)
    All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : Transfected HEK-293T whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    5% SDS-PAGE

  • Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)
    Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)

    Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) protein (62.5-4000 ng/mL). Coated protein probed ab281306 at 1 µg/mL. HRP-Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.

  • Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)
    Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)

    Mock and transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) Spike S1 stained (green) by ab281306 at 1/1000 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue).

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)
    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)
    All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : SARS-CoV Spike transfected HEK-293T whole cell extracts
    Lane 3 : SARS-CoV Spike (COVID-19) transfected HEK-293T whole cell extracts
    Lane 4 : MERS-CoV Spike transfected HEK-293T whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    5% SDS-PAGE

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)
    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)
    Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306) at 1/20000 dilution + SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active)

    Secondary
    HRP-conjugated anti-rabbit IgG antibody


    12% SDS-PAGE

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)
    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)
    All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306) at 1/5000 dilution

    Lane 1 : Non-transfected Vero E6 whole cell extracts
    Lane 2 : SARS-CoV-2 (Covid-19) transfected Vero E6 whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    5% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)

    Paraffin-embedded infected lung tissue derived from the AdV-hACE2-transduced BALB/c mice model. SARS-CoV-2 (COVID-19) Spike RBD protein stained by IgG control (left) and ab281306 at 1/1000 dilution (right) in immunohistochemical analysis. Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 20 minutes.

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