Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Plexin B2/MM1 antibody [EPR9965] - BSA and Azide free (ab251169)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9965] to Plexin B2/MM1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Plexin B2/MM1 antibody [EPR9965] - BSA and Azide free
    See all Plexin B2/MM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9965] to Plexin B2/MM1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293 and HeLa whole cell lysates; human brain tissue lysate

  • General notes

    Ab251169 is the carrier-free version of ab193355. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251169 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] - BSA and Azide free (ab251169)
    Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] - BSA and Azide free (ab251169)
    All lanes : Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355) at 1/5000 dilution

    Lane 1 : Wild-type HEK-293 whole cell lysate
    Lane 2 : Plexin B2/MM1 knockout HEK-293 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : Human brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 204 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab193355 observed at 80 kDa. Red - loading control, ab7291, observed at 50 kDa.

    ab193355 was shown to specifically react with in wild-type HEK-293 cells as signal was lost in PLXNB2 knockout cells. Wild-type and PLXNB2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab193355 and ab7291 (Mouse anti-Tubulin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193355).

  • Anti-Plexin B2/MM1 antibody [EPR9965] - BSA and Azide free (ab251169)
    Anti-Plexin B2/MM1 antibody [EPR9965] - BSA and Azide free (ab251169)

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