Anti-Plexin B2/MM1 antibody [EPR9965] - BSA and Azide free (ab251169)
![Anti-Plexin B2/MM1 antibody [EPR9965] - BSA and Azide free (ab251169)](https://www.abcam.com/ps/products/251/ab251169/Images/ab251169-368363-anti-plexin-b2-antibody-epr9965-western-blot-knockout-hek-293-plxnb2-hela-human-brain-human-lysates.jpg "Anti-Plexin B2/MM1 antibody [EPR9965] - BSA and Azide free (ab251169)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9965] to Plexin B2/MM1 - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Plexin B2/MM1 antibody [EPR9965] - BSA and Azide free
See all Plexin B2/MM1 primary antibodies -
Description
Rabbit monoclonal [EPR9965] to Plexin B2/MM1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293 and HeLa whole cell lysates; human brain tissue lysate
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General notes
Ab251169 is the carrier-free version of ab193355. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251169 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR9965 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] - BSA and Azide free (ab251169)All lanes : Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355) at 1/5000 dilution
Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : Plexin B2/MM1 knockout HEK-293 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 204 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab193355 observed at 80 kDa. Red - loading control, ab7291, observed at 50 kDa.
ab193355 was shown to specifically react with in wild-type HEK-293 cells as signal was lost in PLXNB2 knockout cells. Wild-type and PLXNB2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab193355 and ab7291 (Mouse anti-Tubulin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193355).
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![Anti-Plexin B2/MM1 antibody [EPR9965] - BSA and Azide free (ab251169)](https://www.abcam.com/ps/products/251/ab251169/Images/ab251169-2-benefits-of-recombinant-antibodies.png)
Anti-Plexin B2/MM1 antibody [EPR9965] - BSA and Azide free (ab251169)
