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Neuroscience Neurology process Growth and Development Axonal Guidance Proteins

Anti-SEMA3D antibody [EPR13458(B)] - BSA and Azide free (ab250177)

Anti-SEMA3D antibody [EPR13458(B)] - BSA and Azide free (ab250177)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13458(B)] to SEMA3D - BSA and Azide free
  • Suitable for: WB, Flow Cyt (Intra)
  • Reacts with: Human

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Overview

  • Product name

    Anti-SEMA3D antibody [EPR13458(B)] - BSA and Azide free
    See all SEMA3D primary antibodies
  • Description

    Rabbit monoclonal [EPR13458(B)] to SEMA3D - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt (Intra)more details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250177 is the carrier-free version of ab180174.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13458(B)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration

Images

  • Flow Cytometry - Anti-SEMA3D antibody [EPR13458(B)] - BSA and Azide free (ab250177)
    Flow Cytometry - Anti-SEMA3D antibody [EPR13458(B)] - BSA and Azide free (ab250177)
    This data was developed using ab180174, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized MCF7 cells labeling SEMA3D using ab180174 at 1/10 (red) or a rabbit IgG (negative) (green).
  • Western blot - Anti-SEMA3D antibody [EPR13458(B)] - BSA and Azide free (ab250177)
    Western blot - Anti-SEMA3D antibody [EPR13458(B)] - BSA and Azide free (ab250177)
    All lanes : Anti-SEMA3D antibody [EPR13458(B)] - C-terminal (ab180174) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : Fetal brain lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : Fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 90 kDa



    This data was developed using ab180174, the same antibody clone in a different buffer formulation.

     

  • Anti-SEMA3D antibody [EPR13458(B)] - BSA and Azide free (ab250177)
    Anti-SEMA3D antibody [EPR13458(B)] - BSA and Azide free (ab250177)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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