Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein or recombinant SARS-CoV spike S1 subunit protein (62.5-4000 ng/mL). Coated protein probed with ab281307 at 1 μg/mL. HRP-Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.

Indirect ELISA analysis was performed by coating plate with 50 µL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein at concentrations ranging from 0.0625 µg/mL to 4 µg/mL. The coated protein is detected with ab281307 at 1 µg/mL. HRP-Rabbit IgG antibody at 1/10000 dilution was used to detect the primary antibody.

Mock and SARS-CoV-2 Spike-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. ab281307 at 1/1000 dilution by immunofluorescent analysis. Fluoroshield with DAPI (blue).

Sandwich ELISA detection of non-transfected and SARS-CoV-2 spike (full length) transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts using a SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody at 5 µg/mL of capture antibody and ab281307 as detection antibody at 1 µg/mL. HRP-Rabbit IgG antibody at 1/10000 dilution was used to detect the primary antibody

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with ab281307) (2.4*10⁻⁶ ~ 20 nM). HRP-Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
EC50: 0.117 nM

All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL1] - BSA and Azide free (ab281307) at 1/5000 dilution

Lane 1 : Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : SARS-CoV Spike transfected HEK-293T whole cell extracts
Lane 3 : SARS-CoV-2 (Covid-19) Spike transfected HEK-293T whole cell extracts
Lane 4 : MERS-CoV Spike transfected HEK-293T whole cell extracts

Lysates/proteins at 30 µg per lane.

Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody

5% SDS-PAGE

All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL1] - BSA and Azide free (ab281307) at 1/5000 dilution

Lane 1 : Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : SARS-CoV Spike transfected HEK-293T whole cell extracts

Lysates/proteins at 30 µg per lane.

Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody

5% SDS-PAGE

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (4000-62.5 ng/mL). Coated protein was probed with ab281307 at 1 μg/mL. HRP-Rabbit IgG antibody at 1/10000 dilution was used to detect bound primary antibody.