Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC, Flow Cyt (Intra), IP, ELISA

Overview

  • Product name

    Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free
    See all SARS-CoV-2 nucleocapsid protein primary antibodies

  • Description

    Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt (Intra)
    Recombinant fragment
    ICC
    Recombinant fragment
    IHC-P
    Recombinant fragment
    IP
    Recombinant fragment
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with myc-His-tag® SARS-CoV-2 Nucleocapsid protein; SARS-CoV-2 Nucleocapsid Recombinant Protein; SARS-CoV-2 infected Vero. IHC-P: HEK-293T transfected with myc-His-tag® SARS-CoV-2. ICC: 293T transfected with myc-His-tag® SARS-CoV-2; Vero cells after infecting with SARS-CoV-2 virus for 48 h. Flow cyt: HEK-293T transfected with myc-His-tag® SARS-CoV-2. IP: HEK-293T transfected with myc-His-tag® SARS-CoV-2.

  • General notes

    At the moment, this product is only available for our customers based in the People's Republic of China. We are expecting to make his product available to our customers globally in Spring 2021.

    This antibody is developed with the collaboration of Dr. Hangping Yao, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases in Zhejiang University, School of Medicine.

    ab280201 is the carrier-free version of ab271180. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280201 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney) transfected with SARS-CoV-1 Nucleocapsid protein expression vector containing a myc-His-tag®, whole cell lysate
    Lane 3 : HEK-293T (human embryonic kidney) transfected with MERS Nucleocapsid proteinexpression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Observed band size: 51 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab271180, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time 7.75 seconds

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] (ab271180) at 1/1000 dilution

    Lane 1 : SARS-CoV-2 Nucleocapsid Recombinant Protein at 0.1 µg
    Lane 2 : SARS-CoV-2 infected Vero for 48 hours
    Lane 3 : Uninfected Vero

    Observed band size: 51 kDa why is the actual band size different from the predicted?



    This data was developed using ab271180, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Data was kindly provided by Dr. Hangping Yao, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases in Zhejiang University, School of Medicine.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)

    This data was developed using ab271180, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag® (Panel A) 
    HEK-293T transfected with SARS-CoV-1 Nucleocapsid protein expression vector containing a myc-His-tag® (Panel B) HEK-293T transfected with MERS Nucleocapsid protein expression vector containing a myc-His-tag® (Panel C) HEK-293T transfected with control vector containing a myc-His-tag® (Panel D) with 271180 at 1/4000 dilution followed by ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on (A) HEK-293T transfected with a SARS-CoV-2 Nucleocapsid protein expression vector is observed. No staining on (B) HEK-293T transfected with a SARS-CoV-1 Nucleocapsid protein expression vector, (C) HEK-293T transfected with MERS Nucleocapsid protein expression vector and (D) HEK-293T transfected with empty vector. The section was incubated with ab271180 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)

    This data was developed using ab271180, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded Human lung labeling SARS-CoV-2 with ab271180 at 1/4000 dilution followed by ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: No staining on human lung. The section was incubated with ab271180 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)

    This data was developed using ab271180, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded Mouse lung labeling SARS-CoV-2 with ab271180 at 1/4000 dilution followed by ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: No staining on Mouse lung. The section was incubated with ab271180 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)

    This data was developed using ab271180, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded Rat lung labeling SARS-CoV-2 with ab271180 at 1/4000 dilution followed by ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control: No staining on Rat lung. The section was incubated with ab271180 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
    Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)

    This data was developed using ab271180, the same antibody clone in a different buffer formulation. 

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100-permeabilized 293T cells transfected with myc-tagged SARS-CoV-2 Nucleocapsid protein expression vector labelling SARS-CoV-2 with ab271180 at 1/1000 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (Red). ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) was used to counterstain Myc tag at 1/200 dilution (Green). The nuclear counterstain was DAPI (Blue). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged SARS-CoV-2 Nucleocapsid protein expression vector.

     

  • Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
    Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)

    This data was developed using ab271180, the same antibody clone in a different buffer formulation. 

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100-permeabilized Vero cells labelling SARS-CoV-2 with ab271180 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/500 dilution. Fluorescent image showing positve staining in Vero cells after infecting with SARS-CoV-2 virus for 48 h. Nuclear counter stain DAPI. 
    Two indenpendent optical fields were captured for imaging at each given antibody dilution.

    This piece of data was kindly provided by Dr. Hangping Yao, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases in Zhejiang University, School of Medicine

  • Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
    Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)

    This data was developed using ab271180, the same antibody clone in a different buffer formulation. 

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100-permeabilized 293T cells transfected with myc-tagged SARS-CoV-1 and MERS Nucleocapsid protein labelling SARS-CoV-2 with ab271180 at 1/1000 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution. ab202008 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 488) was used to counterstain Myc tag at 1/200 dilution (Green). The nuclear counterstain was DAPI (Blue). Confocal image showing no staining in 293T cells transfected with myc-tagged SARS-CoV-1 and MERS Nucleocapsid protein, which are negative controls for SARS-CoV-2.

     

  • Flow Cytometry (Intracellular) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
    Flow Cytometry (Intracellular) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)

    This data was developed using ab271180, the same antibody clone in a different buffer formulation. 

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney) transfected with myc-tagged SARS-CoV-2 Nucleocapsid protein expression construct  labelling SARS-CoV-2 with ab271180 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. 

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077 at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
    Flow Cytometry (Intracellular) - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)

    This data was developed using ab271180, the same antibody clone in a different buffer formulation. 

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney) transfected with myc-tagged SARS-CoV-1 Nucleocapsid protein expression construct (Left)/ HEK-293T (human embryonic kidney) transfected with myc-tagged MERS Nucleocapsid protein expression construct (Right). 

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077 at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
    Immunoprecipitation - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)

    This data was developed using ab271180, the same antibody clone in a different buffer formulation. 

    SARS-CoV-2 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag®, whole cell lysate 5 µg with ab271180 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267373 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HEK-293T (human embryonic kidney) transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag® whole cell lysate 5 μg

    Lane 2: ab271180 IP in HEK-293T transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag® whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271180 in HEK-293T transfected with SARS-CoV-2 Nucleocapsid protein expression vector containing a myc-His-tag® whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3.25 seconds.

     

  • ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
    ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)

    This data was developed using ab271180, the same antibody clone in a different buffer formulation. 

    SARS-CoV-2 infected Vero cell supernatant was used for coating at 1/10 dilution. Primary antibody - ab271180 concentration range 5000~0 ng /ml. Secondary antibody Goat Anti-Rabbit IgG H&L (HRP) at 1/10000 dilution


    Data was kindly provided by Dr. Hangping Yao, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases in Zhejiang University, School of Medicine.

  • ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)
    ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (ab280201)

    This data was developed using ab271180, the same antibody clone in a different buffer formulation. 

    SARS-CoV-2 Nucleocapsid Recombinant Protein，SARS-CoV-1 Nucleocapsid Recombinant Protein，MERS Nucleocapsid Recombinant Protein at 1000 ng/ml concentration.

    Primary antibody - ab271180 concentration range 1000~0 ng /ml. Secondary antibody Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2000 dilution. 

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