Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765)
![Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765)](https://www.abcam.com/ps/products/75/ab75765/Images/ab75765-90820-anti-topoisomerase-ii-alpha-phospho-s1106-antibody-ep2722-western-blot.gif "Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2722] to Topoisomerase II alpha (phospho S1106)
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722]
See all Topoisomerase II alpha primary antibodies -
Description
Rabbit monoclonal [EP2722] to Topoisomerase II alpha (phospho S1106) -
Host species
Rabbit -
Specificity
ab75765 detects Topoisomerase II alpha phosphorylated on Serine 1106. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
A phospho specific peptide corresponding to residues surrounding Serine 1106 of human Topoisormerase II alpha
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Positive control
- HeLa whole cell lysate (ab150035), untreated or treated with AP and colonic adenocarcinoma tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2722 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765)All lanes : Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765) at 1/500 dilution
Lane 1 : HeLa cell lysates, untreated
Lane 2 : HeLa cell lysates, treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 174 kDa
Observed band size: 174 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765)](https://www.abcam.com/ps/products/75/ab75765/Images/ab75765-90819-anti-topoisomerase-ii-alpha-phospho-s1106-antibody-ep2722-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765)ab75765 at 1/100 dilution staining Topoisomerase II alpha in colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765)](https://www.abcam.com/ps/products/75/ab75765/Images/ab75765-3-benefits-of-recombinant-antibodies.png)
Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765)
