Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343)
Suitable for: IHC-P, WB, ICC
Reacts with: Human

Product name

Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y]
See all Topoisomerase II alpha primary antibodies
Description

Rabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343)
Host species

Rabbit
Specificity

ab52853 only detects Topoisomerase II alpha phosphorylated on Threonine 1343.

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topoisomerase II alpha.
Positive control
- WB: HeLa cell lysates. IHC-P: Human cervical carcinoma tissue. ICC: HeLa cells
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP1054Y
Isotype

IgG
Research areas

Western blot - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)

All lanes : Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853) at 1/1000 dilution

Lane 1 : HeLa cell lysate treated with AP
Lane 2 : HeLa cell lysate untreated

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 174 kDa
Observed band size: 174 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)

ab52853 at 1/100 dilution staining Topoisomerase II alpha in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)

Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Topoisomerase II alpha with purified ab52853 at 1/50 dilution (4 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.14 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.
Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com