Anti-Topoisomerase II alpha (phospho S1525) antibody (ab138683)
Key features and details
- Rabbit polyclonal to Topoisomerase II alpha (phospho S1525)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Topoisomerase II alpha (phospho S1525) antibody
See all Topoisomerase II alpha primary antibodies -
Description
Rabbit polyclonal to Topoisomerase II alpha (phospho S1525) -
Host species
Rabbit -
Specificity
ab138683 detects endogenous levels of Topoisomerase II alpha only when phosphorylated at S1525. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic phosphopeptide derived from the C-terminal region of Human Topoisomerase II alpha around the phosphorylation site of S1525.
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Positive control
- RAW 264.7 cell extract, treated with TNF (20ng/ml, 5 mins).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 49% PBS
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab138683 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Topoisomerase II alpha (phospho S1525) antibody (ab138683)Anti-Topoisomerase II alpha (phospho S1525) antibody (ab138683) at 1/500 dilution + RAW 264.7 cell extracts, treated with TNF (20ng/ml, 5 mins) at 30 µg
Predicted band size: 174 kDa
