Anti-smooth muscle Myosin heavy chain 11 antibody - ChIP Grade (ab195481)
Key features and details
- Rabbit polyclonal to smooth muscle Myosin heavy chain 11 - ChIP Grade
- Suitable for: ChIP-sequencing, WB, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-smooth muscle Myosin heavy chain 11 antibody - ChIP Grade
See all smooth muscle Myosin heavy chain 11 primary antibodies -
Description
Rabbit polyclonal to smooth muscle Myosin heavy chain 11 - ChIP Grade -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human smooth muscle Myosin heavy chain 11 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. (2 peptides used, aa 50 - 100)
Database link: P35749 -
Positive control
- Chromatin prepared from ME-1 cells; ME-1 nuclear extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody - ChIP Grade (ab195481)Lane 1 : CBFb antibody at 1/1000 dilution
Lane 2 : Anti-smooth muscle Myosin heavy chain 11 antibody - ChIP Grade (ab195481) at 1/1000 dilution
All lanes : ME-1 nuclear extract
Predicted band size: 227 kDaThe antibody was diluted in TBS-Tween containing 5% skimmed milk.
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ChIP - Anti-smooth muscle Myosin heavy chain 11 antibody - ChIP Grade (ab195481)ChIP results obtained with ab195481 directed against smooth muscle Myosin heavy chain 11.
ChIP assays were performed using ME-1 cells, ab195481 and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 µl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.
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ChIP-sequencing - Anti-smooth muscle Myosin heavy chain 11 antibody - ChIP Grade (ab195481)ChIPseq results obtained with ab195481 directed against smooth muscle Myosin heavy chain 11.
ChIP was performed. The IP’d DNA from 6 ChIP’s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.
