Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541)
Key features and details
- Rabbit polyclonal to smooth muscle Myosin heavy chain 11
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-smooth muscle Myosin heavy chain 11 antibody
See all smooth muscle Myosin heavy chain 11 primary antibodies -
Description
Rabbit polyclonal to smooth muscle Myosin heavy chain 11 -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 82% homology with non-muscle myosin 9. The cross-reactivity with this protein has not been confirmed experimentally. -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Orangutan
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Immunogen
Synthetic peptide corresponding to Human smooth muscle Myosin heavy chain 11 aa 550-650 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93494) -
Positive control
- WB: Bladder tissue; Heart tissue; PC3 whole cell; HUVEC whole cell. ICC: SV40LT-SMC cell line.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Images
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Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541)
ICC/IF image of ab82541 stained SV40LT-SMC cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82541, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541)All lanes : Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541) at 1 µg/ml
Lane 1 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lane 2 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 3 : Human bladder tissue lysate - soluble fraction (female, 45 years) (ab44654)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 227 kDa
Observed band size: 227 kDa
Additional bands at: 49 kDa, 64 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
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Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541) Image courtesy of an anonymous Abreview.Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541) at 1/2000 dilution + whole cell lysate prepared from human PASMC, P6 at 300 µg
Secondary
HRP conjugated goat anti-rabbit polyclonal at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 227 kDa
Observed band size: 227 kDa
Additional bands at: 100 kDa, 200 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 seconds
