Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5336(B)] to smooth muscle Myosin heavy chain 11 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt (Intra), IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free
    See all smooth muscle Myosin heavy chain 11 primary antibodies

  • Description

    Rabbit monoclonal [EPR5336(B)] to smooth muscle Myosin heavy chain 11 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cyt (Intra), IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: Human ovary and prostate tissues; WB: Human bladder, artery and testis tissue lysates; Flow Cyt (intra): 293 cells; ICC: Human smooth muscle and A673 cells.

  • General notes

    ab240069 is the carrier-free version of ab133567.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue sections labeling smooth muscle Myosin heavy chain 11 with purified ab133567 at 1/1000 dilution (0.12 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133567).
  • Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)
    Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)
    Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567) at 1/5000 dilution (purified) + Human testis lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 227 kDa
    Observed band size: 227 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133567).

  • Immunocytochemistry - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)
    Immunocytochemistry - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)

    Immunocytochemistry/ Immunofluorescence analysis of A-673 (Human muscle Ewing's Sarcoma) cells labeling smooth muscle Myosin heavy chain 11 with purified ab133567 at 1/50 dilution (2.34 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488 , ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133567).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)

    Immunohistochemical analysis of paraffin-embedded Human ovary tissue labelling smooth muscle Myosin heavy chain 11 with unpurified, ab133567 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133567).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)
    Flow Cytometry - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)

    Flow cytometry analysis of 293 (Human embryonic kidney epithelial cell) cells labeling smooth muscle Myosin heavy chain 11 (red) with purified ab133567 at a 1/20 dilution (10ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133567).

  • Immunocytochemistry - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)
    Immunocytochemistry - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069) This image is courtesy of an anonymous abreview.

    Unpurified ab133567 staining smooth muscle Myosin heavy chain 11 in Human smooth muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized and blocked in 1% serum, 0.1%triton, 0.1% BSA in PBS. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. A Goat anti-rabbit IgG Alexa 488 (green) was used as the secondary antibody, and DAPI was used to stain cell nuclei (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133567).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)

    Immunohistochemical analysis of paraffin-embedded Human prostate tissue labelling smooth muscle Myosin heavy chain 11 with unpurified ab133567 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133567).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)
    Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] - BSA and Azide free (ab240069)

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