Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling smooth muscle Myosin heavy chain 11 with ab224804 at 1/100 dilution (1.34 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the smooth muscle cells in human colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224804 for 10 mins at room temperature.

This image was generated using ab224804, the same clone, but with a different buffer formulation.

Immunohistochemistry (Frozen) analysis of rat small intestine tissue section labeling smooth muscle Myosin heavy chain 11 with purified ab224804 at 1/500 (1.8 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224804).

Flow cytometry analysis of C2C12 (Mouse myoblasts myoblast) labeling smooth muscle Myosin heavy chain 11 with purified ab224804 at 1/80 dilution (1.12 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224804).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling smooth muscle Myosin heavy chain 11 with ab224804 at 1/100 dilution (1.34 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the smooth muscle cells in rat colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224804 for 10 mins at room temperature.

This image was generated using ab224804, the same clone, but with a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling smooth muscle Myosin heavy chain 11 with ab224804 at 1/100 dilution (1.34 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the smooth muscle cells in mouse stomach, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224804 for 10 mins at room temperature.

This image was generated using ab224804, the same clone, but with a different buffer formulation.

Formalin-fixed, paraffin-embedded human ovary tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224804).

Immunohistochemistry (Frozen) analysis of mouse small intestine tissue section labeling smooth muscle Myosin heavy chain 11 with purified ab224804 at 1/500 (1.8 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224804).

Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224804).

Formalin-fixed, paraffin-embedded human prostate tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224804).

Formalin-fixed, paraffin-embedded human colon tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224804).

Formalin-fixed, paraffin-embedded human small intestine tissue stained for smooth muscle Myosin heavy chain 11 using ab224804 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224804).