Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MYO1C antibody [EPR14771] - BSA and Azide free (ab251196)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14771] to MYO1C - BSA and Azide free
  • Suitable for: WB, ICC, IHC-P, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MYO1C antibody [EPR14771] - BSA and Azide free
    See all MYO1C primary antibodies

  • Description

    Rabbit monoclonal [EPR14771] to MYO1C - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, IHC-P, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251196 is the carrier-free version of ab194828.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MYO1C antibody [EPR14771] - BSA and Azide free (ab251196)
    Western blot - Anti-MYO1C antibody [EPR14771] - BSA and Azide free (ab251196)
    All lanes : Anti-MYO1C antibody [EPR14771] (ab194828) at 1/20000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HT1080 cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 122 kDa



    This data was developed using ab194828, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO1C antibody [EPR14771] - BSA and Azide free (ab251196)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO1C antibody [EPR14771] - BSA and Azide free (ab251196)
    This data was developed using ab194828, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MYO1C with ab194828 at 1/200 dilution (5μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab194828. Cytoplasm staining on Human tonsil was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-MYO1C antibody [EPR14771] - BSA and Azide free (ab251196)
    Immunocytochemistry - Anti-MYO1C antibody [EPR14771] - BSA and Azide free (ab251196)
    This data was developed using ab194828, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of A431 cells labeling MYO1C with ab194828 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm and nuclear on A431 cell line was observed.
  • Western blot - Anti-MYO1C antibody [EPR14771] - BSA and Azide free (ab251196)
    Western blot - Anti-MYO1C antibody [EPR14771] - BSA and Azide free (ab251196)
    All lanes : Anti-MYO1C antibody [EPR14771] (ab194828) at 1/2000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 122 kDa



    This data was developed using ab194828, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-MYO1C antibody [EPR14771] - BSA and Azide free (ab251196)
    Flow Cytometry - Anti-MYO1C antibody [EPR14771] - BSA and Azide free (ab251196)
    This data was developed using ab194828, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa cells labelling MYO1C (red) with purified ab194828 at dilution of 1/80. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
  • Anti-MYO1C antibody [EPR14771] - BSA and Azide free (ab251196)
    Anti-MYO1C antibody [EPR14771] - BSA and Azide free (ab251196)

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