Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free (ab278086)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23994-103] to Calcium-independent Phospholipase A2/PLA2G6 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free
    See all Calcium-independent Phospholipase A2/PLA2G6 primary antibodies

  • Description

    Rabbit monoclonal [EPR23994-103] to Calcium-independent Phospholipase A2/PLA2G6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse testis tissue; Rat testis tissue lysates; F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate; C6 (rat glial tumor glial cell), whole cell lysate. IHC-P: Mouse testis; Rat testis.

  • General notes

    ab278086 is the carrier-free version of ab259950.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free (ab278086)
    Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free (ab278086)
    All lanes : Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950) at 1/1000 dilution

    Lane 1 : Mouse testis tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Mouse lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 89 kDa
    Observed band size: 36, 88 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab259950, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 29109765)

    Low expression: brain, lung (PMID:10336645)

    Exposure time: 10 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free (ab278086)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free (ab278086)

    This data was developed using ab259950, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Calcium-independent Phospholipase A2/PLA2G6 with ab259950 at 1/100 (8.18 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse testis (PMID: 9079687). The section was incubated with ab259950 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free (ab278086)
    Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free (ab278086)
    All lanes : Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950) at 1/1000 dilution

    Lane 1 : Rat testis tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 89 kDa
    Observed band size: 36, 88 kDa why is the actual band size different from the predicted?



    This data was developed using ab259950, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID:29109765)

    Low expression: brain, lung (PMID:10336645)

    Exposure time: 10 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free (ab278086)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free (ab278086)

    This data was developed using ab259950, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Calcium-independent Phospholipase A2/PLA2G6 with ab259950 at 1/100 (8.18 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat testis (PMID: 9079687). The section was incubated with ab259950 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free (ab278086)
    Western blot - Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] - BSA and Azide free (ab278086)
    All lanes : Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody [EPR23994-103] (ab259950) at 1/1000 dilution

    Lane 1 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate
    Lane 2 : C6 (rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 89 kDa
    Observed band size: 88 kDa why is the actual band size different from the predicted?



    This data was developed using ab259950, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The blot was developed using a higher sensitivity ECL substrate.

     Exposure time: 3 minutes

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